Target genes, identified by their phenotypic effect, can be located by positional cloning. Current genetic maps in livestock species lack the necessary detail for direct application in these projects. Fine maps of each target region must be developed using a variety of strategies. These include chromosome specific libraries and comparative mapping. A physical map of the region is generated by identifying overlapping clones containing large DNA inserts. All genes from the region are sought and candidate genes identified. The resources being developed in livestock projects, including the search for the Booroola (FecB) locus, and complete expression maps for the human genome will greatly facilitate future positional cloning projects in livestock.
Proceedings of the New Zealand Society of Animal Production, Volume 57, , 302-304, 1997
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