Recently a bioassay for human FSH has been described based on FSH stimulation of cyclic AMP production by a Chinese hamster ovary (CHO/FSH-R) cell line stably expressing the human FSH receptor (FSH-R cells). In this study, the viability of this bioassay for measuring ovine FSH was examined. Freshly thawed FSH-R cells were bulked up in culture, and aliquots of 250,000 cells/well dispensed into 48 well culture dishes and incubated overnight at 37°C. The assay culture media was then replaced with 0.25 ml fresh media (α-MEM + 0.1% BSA + 0.25 mM 3-isobutyl-1-methyl-xanthine) containing varying doses of FSH preparations, and the cells incubated for 4 h at 37°C. The assay culture media was then removed and stored frozen at -20°C until assayed for cyclic AMP by radio immunoassay.
Proceedings of the New Zealand Society of Animal Production, Volume 57, , 243-245, 1997
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