The use of unfrozen, diluted bovine semen for in vitro fertilisation (IVF) was investigated. In vitro matured bovine oocytes were incubated with various (0.125 - 4.0 x 10 6/ml) concentrations of washed sperm. Penetration rates ranged from 21% at low sperm concentrations to 80-100% above 1 x 10 6 sperm/ml (P<0.01). Unfrozen sperm gave better penetration rates than frozen-thawed sperm. Oocytes were incubated with washed sperm (1 x 10 6/ml) in increasing dilutions of caprogen extender. Sperm penetration was significantly inhibited at dilutions of 10-fold or less (P<0.01). Oocytes were inseminated with washed sperm (1 x 10 6/ml) at various concentrations (0.50 ug/ml) of heparin. No significant differences were found.
Proceedings of the New Zealand Society of Animal Production, Volume 51, , 15-24, 1991
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