@conference {40771,
	title = {Manipulation of gene expression in transgenic mice using ribozymes},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {56},
	year = {1996},
	month = {Jan},
	pages = {42-44},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {This study describes the creation and analysis of transgenic mice  carrying a ribozyme or RNA enzyme that specifically targets the bovine a- lactalbumin (a-lac) mRNA transcript. Using a MMTV-LTR promoter-based  construct, mammary specific high-level expression of a bovine a-lac- targeted ribozyme was obtained in three out of twelve lines of transgenic  mice created. When crossed with mice transgenic for the bovine a-lac gene  (0.4 mg of a-lac.ml-1 of milk), expression of the ribozyme resulted in a  reduction in the levels of the target mRNA to 50\% of that observed in the  non-ribozyme transgenic littermate controls. This activity was specific to the  bovine a-lac transcript as no reduction in the levels of the endogenous  murine a-lac mRNA was observed. These results demonstrate the feasibility  of ribozyme-mediated down-regulation of highly-expressed transcripts in  transgenic animals. },
	issn = {1176-5283},
	author = {L{\textquoteright}Huillier PJ and Vilotte JL}
}
