@conference {40623,
	title = {Evidence for a diffusible factor influencing the switching of ovine and bovine milk gene expression leads to identification of factors},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {54},
	year = {1994},
	month = {Jan},
	pages = {303-306},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Our earlier <I>in situ</I> hybridisation studies have shown heterogeneous  
expression of milk protein genes.  At some sites, a sharp demarcation of  
mRNA expression is seen at the basement membrane between {\textquoteright}active{\textquoteright} and  
{\textquoteright}inactive{\textquoteright} tissue, while at other nearby sites, a reciprocal gradient of mRNA  
expression about 10 cells wide is seen.  The alveoli in these latter {\textquoteright}inactive{\textquoteright}  
sites contain leucocytes which indicates that they have been {\textquoteright}inactive{\textquoteright} longer  
than the former.  This suggests a factor could be diffusing between {\textquoteright}inactive{\textquoteright}  
and {\textquoteright}active{\textquoteright} tissues and influencing milk gene expression.  We used a  
consensus oligomer corresponding to the linkers between the fingers of zinc  
finger DNA binding proteins in a polymerase chain reaction performed on  
cDNA from {\textquoteright}active{\textquoteright} versus {\textquoteright}inactive{\textquoteright} mammary mRNA.  Differences and  
similarities were observed in the sizes of the DNA products from each sample.   
Several of the bands present in the {\textquoteright}active{\textquoteright} cDNA and absent from the  
{\textquoteright}inactive{\textquoteright} cDNA were cloned and some were found to have homologies to zinc  
finger and other DNA binding proteins.  It is possible that we have partially  
isolated a component of the pathway that maintains lactation. },
	issn = {1176-5283},
	author = {Molenaar AJ and Musgrave DR and Wilkins RJ}
}
@conference {39913,
	title = {The development and application of ELISA detection of Lolium endophyte in ryegrass staggers research},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {44},
	year = {1984},
	month = {Jan},
	pages = {185-188},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {To apportion the intake of metabolisable energy (MEI) used for synthesis of lean and fat tissue, current knowledge of substrate oxidation and substrate utilisation for biosynthetic reactions has been combined with estimates of fat and protein turnover to develop models of energy metabolism and nutrient fluxes in a lean 20 kg male and a fat 40 kg female sheep, each gaining 200 g/d. When the most efficient pathways and lowest turnover rates are employed the biosynthetic reactions account for 38 and 34\% of MEI in the lean and fat animals. These values rise to 45 and 43\% of MEI respectively when higher estimates of turnover and less efficient pathways are used in the calculations. Hormonal regulations of metabolite utilisation is believed responsible for such differences but hitherto, only glucose-insulin system has been quantitated. Two techniques used for its quantitation are described to provide examples of the methods needed to elucidate the control of nutrient utilisation and ultimately body composition in ruminants.},
	issn = {1176-5283},
	author = {Musgrave DR and Fletcher LR}
}
