@conference {40779,
	title = {Oestrogenic effects on in vitro co-culture of mammary explants and epithelial cells},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {56},
	year = {1996},
	month = {Jan},
	pages = {62-64},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Mechanisms of oestrogen stimulation of mammary development were  examined using ewe lambs treated with oestradiol (0.1mg/kg BW/day for 7  days; n=5) or controls (n=5). Mammary explants were incubated with [3H]- thymidine to determine effects of oestrogen on epithelial cell proliferation.  Also, explants of mammary stroma and parenchyma were co-cultured with a  mammary epithelial cell line (MAC-T) to determine the ability of the  explants to stimulate MAC-T growth. Oestrogen increased proliferation of  ewe mammary epithelial cells (P < 0.02). In co-cultures, addition of  mammary explants increased growth of MAC-T cells (P < 0.01), but there  was no effect or interaction of tissue type (stroma vs. parenchyma) or  oestrogen (P > 0.10). Proliferation of mammary explants was negatively  correlated with growth of MAC-T cells in co-cultures (r = -0.41; P < 0.07).  These results confirm the importance of oestrogen and mammary stroma in  regulating mammary epithelial proliferation but fail to show a requirement  for mammary stroma to mediate oestrogenic effects on epithelial cells. },
	issn = {1176-5283},
	author = {Ellis S and McFadden TB and Akers RM}
}
