@conference {41242,
	title = {Evaluation of an oestrone sulphate dipstick immunoassay for pregnancy testing mares},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {61},
	year = {2001},
	month = {Jan},
	pages = {45-47},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Christchurch},
	abstract = {Serum samples from 213 mares were received for routine pregnancy testing and analysed by a conventional oestrone sulphate (OS) enzymeimmunoassay (EIA) and a new OS dipstick immunoassay. OS concentrations, measured by EIA, in 122 of the samples were <10 ng/ml, indicating the mares were not pregnant. The same 122 samples also returned a {\textquoteleft}not pregnant{\textquoteleft} diagnosis when analysed by the dipstick immunoassay. The remaining 91 samples returned diagnoses of {\textquoteleft}pregnant{\textquoteleft} by the OS EIA, i.e., OS concentrations were >20 ng/ml. Of these, 89 also returned a {\textquoteleft}pregnant{\textquoteleft} diagnosis by the dipstick assay, but 2 returned a {\textquoteleft}not pregnant{\textquoteleft} diagnosis. Both these mares were found to be pregnant on follow up, indicating that the dipstick test had returned a false negative diagnosis in each instance. These results show that there is 100\% agreement between the conventional OS EIA and the new dipstick assay in diagnosing {\textquoteleft}non pregnancy{\textquoteleft}. However, the dipstick assay may return a small proportion (2.2\%) of false negative diagnoses relative to the EIA. Overall, the new dipstick immunoassay offers a practical alternative to the OS EIA for diagnosing pregnancy status in mares. },
	issn = {1176-5283},
	author = {Stewart J and Henderson K}
}
@conference {40947,
	title = {The measurement of ovine follicle stimulating hormone (FSH) bioactivity utilizing a recombinant human FSH receptor bioassay.},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {57},
	year = {1997},
	month = {Jan},
	pages = {243-245},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Recently a bioassay for human FSH has been described based on FSH stimulation of cyclic AMP production by a Chinese hamster ovary (CHO/FSH-R) cell line stably expressing the human FSH receptor (FSH-R cells). In this study, the viability of this bioassay for measuring ovine FSH was examined. Freshly thawed FSH-R cells were bulked up in culture, and aliquots of 250,000 cells/well dispensed into 48 well culture dishes and incubated overnight at 37{\textdegree}C. The assay culture media was then replaced with 0.25 ml fresh media (α-MEM + 0.1\% BSA + 0.25 mM 3-isobutyl-1-methyl-xanthine) containing varying doses of FSH preparations, and the cells incubated for 4 h at 37{\textdegree}C. The assay culture media was then removed and stored frozen at -20{\textdegree}C until assayed for cyclic AMP by radio immunoassay.},
	issn = {1176-5283},
	author = {Young W and Henderson K and Hudson N and Moore L and Albanese C}
}
