@article {3672,
	title = {BRIEF COMMUNICATION: Differential expression of lactoferrin and insulin-like growth factor-1 genes in milk from cows milked once and twice a day},
	journal = {New Zealand Journal of Animal Science and Production},
	volume = {78},
	year = {2018},
	month = {07/2018},
	pages = {109-110},
	address = {Lincoln},
	abstract = {Dairy farming in New Zealand has traditionally utilized a twice a day (TAD) milking system, but milking once a day (OAD) for the
entire lactation is also practiced by a significant proportion of dairy farmers. This study reports on the differential gene expression
in milk from OAD and TAD cows. Three predominant dairy breed group of New Zealand, Holstein-Friesian (F), Jersey (J) and F{\texttimes}J
crossbred (F{\texttimes}J) were included in samples collected from both milking regimens. RNA was extracted from milk from 45 OAD and
42 TAD dairy cows during early lactation, and used to determine lactoferrin (LF) and insulin-like growth factor-1 (IGF-1) gene
expression using qPCR. Compared to TAD cows, cows milked OAD had higher expression of the LF gene (1.38 vs 1.30 folds,
P=0.08) and the IGF-1 gene (1.65 vs 1.48 folds, P=0.09). These results suggest that milking frequency affects the expression of
milk composition genes at early lactation.},
	issn = {1176-5283},
	author = {Gedye K and Notcovich S and CorreaLuna M and Ariyarathne P and Heiser A and Lopez-villalobos N}
}
