@conference {40206,
	title = {Developments in artificial insemination and controlled breeding in dairy cattle and deer in New Zealand},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {50},
	year = {1990},
	month = {Jan},
	pages = {123-134},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {A number of techniques for directly manipulating the mammalian   germline have been established in the mouse. During the past five years   these have been applied to domestic livestock but, so far, only   pronuclear microinjection has proved successful. The considerable   resources required and the low efficiency of the procedure are major   limitations and, worldwide, relatively few laboratories have produced   transgenic livestock. In applying rtransgenic technology to farm   animals, a major effort has gone into the manipulation of growth. This   is a complex trait and progress has been constrained by the inability to   control the expression of transgenes in a precise manner, and by the   lack of basic knowledge at the interface between molecular biology and   physiology. By contrast, the production of biomedical proteins from   transgenic animals involves the manipulation of a relatively simple   trait, milk composition. So far, only relatively low levels of protein   production have been reported for transgenic sheep. However, very high   levels of expression of human a1-antitrypsin have been obtained in   transgenic mice, demonstrating the feasibility for this application of   transgenic animals.    },
	issn = {1176-5283},
	author = {Macmillan KL and Asher GW}
}
