@conference {40241,
	title = {Animal production in the future - a New Zealand response},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {50},
	year = {1990},
	month = {Jan},
	pages = {361-372},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {As a pre-requisite to examine the possibility of using   spermatozoa as a vector for the incorporation of foreign DNA into   livestock, the ability of sperm from sheep, fallow deer and cattle to   bind exogenous DNA was examined.     The DNA was obtained from the plasmid pMK1 linearised with EcORIXhoI and   end-labelled with 32P. Fresh sperm from all species bound DNA with   maximum binding occurring after 1hr of incubation. Frozen sperm bound   less DNA and formaldehyde treated sperm did not bind. Seminal plasma   inhibited binding as did heparin and also high divalent cation   concentrations. Capacitated ram sperm bound more than uncapacitated   (64\% vs 40\%) and uterine fluids from sheep two-days post-estrus did not   interfere with DNA binding to the capacitated ram sperm.     Increasing the concentration of washed capacitated ram sperm from 1 x 10   6/ml to 30 x 10 6/ml and with a DNA concentration of 0.25 mg/ml   decreased the number of molecules of DNA bound from 38,000 to 1,600.   There was wide variation among rams in the percentage of DNA bound after   1hr incubation, ranging from 15-80\% (n=7).     These results suggest that this procedure may be a possible method for   the incorporation of foreign DNA into livestock by intrauterine   insemination.   },
	issn = {1176-5283},
	author = {Fennessy PF}
}
