@conference {40623,
	title = {Evidence for a diffusible factor influencing the switching of ovine and bovine milk gene expression leads to identification of factors},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {54},
	year = {1994},
	month = {Jan},
	pages = {303-306},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Our earlier <I>in situ</I> hybridisation studies have shown heterogeneous  
expression of milk protein genes.  At some sites, a sharp demarcation of  
mRNA expression is seen at the basement membrane between {\textquoteright}active{\textquoteright} and  
{\textquoteright}inactive{\textquoteright} tissue, while at other nearby sites, a reciprocal gradient of mRNA  
expression about 10 cells wide is seen.  The alveoli in these latter {\textquoteright}inactive{\textquoteright}  
sites contain leucocytes which indicates that they have been {\textquoteright}inactive{\textquoteright} longer  
than the former.  This suggests a factor could be diffusing between {\textquoteright}inactive{\textquoteright}  
and {\textquoteright}active{\textquoteright} tissues and influencing milk gene expression.  We used a  
consensus oligomer corresponding to the linkers between the fingers of zinc  
finger DNA binding proteins in a polymerase chain reaction performed on  
cDNA from {\textquoteright}active{\textquoteright} versus {\textquoteright}inactive{\textquoteright} mammary mRNA.  Differences and  
similarities were observed in the sizes of the DNA products from each sample.   
Several of the bands present in the {\textquoteright}active{\textquoteright} cDNA and absent from the  
{\textquoteright}inactive{\textquoteright} cDNA were cloned and some were found to have homologies to zinc  
finger and other DNA binding proteins.  It is possible that we have partially  
isolated a component of the pathway that maintains lactation. },
	issn = {1176-5283},
	author = {Molenaar AJ and Musgrave DR and Wilkins RJ}
}
