@conference {40855,
	title = {Brief Communication: The effect of inorganic ions on ram sperm motility using Computer Assisted Sperm Analysis (CASA)},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {56},
	year = {1996},
	month = {Jan},
	pages = {353-354},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {The role of specific inorganic ions in the maintenance of ram sperm  motility in a chemically defined ram semen diluent (RSD-1) was examined.  A preliminary experiment identified that magnesium was required to  maintain motility. Significant treatment and incubation effects were  observed for all motility parameters (P<.05). RSD-1 (treatment T1) was  modified to provide a series of treatments. Effects measured were the  substitution of MgCl2 (T2) or MgSO4 (T3) and the elimination of KH2PO4  (T4) or NaHCO3 (T5). A total of 10 ejaculates were analysed using the  Hobson Sperm Tracker (HST) over a period of 7 days. Semen was cooled to  15 C after dilution with treatment diluents and reheated to 38 C after 1 hr  and 5 days storage. Motility was measured after incubation at 38 C for 0,  24, and 48 hours. Treatments had a significant (P<0.001) effect on the  velocity parameters and the percentage of motile sperm. Treatments T4 and  T5 had greatest depression in motility parameters with fresh semen while T5  also had an effect with stored sperm. Substitution of the MgSO4 (T3)  reduced the velocity parameters in the fresh semen e.g. mean Curvilinear  velocity (VCL) values (},
	issn = {1176-5283},
	author = {Briggs RM and Upreti GC and Smith JF and Duganzich DM}
}
